SRF anticorps

N° du produit ABIN7126983

Cet anticorps Souris Monoclonal détecte spécifiquement SRF dans WB, ELISA, IP, IF, FACS, Coat et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour SRF anticorps (ABIN7126983)

Antigène: SRF (Serum Response Factor (SRF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SRF est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-SRF anticorps

Specificité: Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human SRF protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Flow Cytometry (1-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U87 or Jurkat cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur SRF

Antigène: SRF (Serum Response Factor (SRF))

Autre désignation: SRF

Sujet: C fos serum response element binding transcription factor, ELK3, ERP, MCM1, SAP2, Serum response factor (SRF), SRF serum response factor c fos serum response element binding transcription factor,Serum Response Element Binding Transcription Factor (SRF)
Cellular localisation: Nucleus. Nucleoplasm.

Poids moléculaire: 40-67kDa

ID gène: 6722, 520140

UniProt: P11831